RAPID METHOD OF COUNTING BACTERIA IN MILK

Rapid Method for Counting Reticulocytes.

Among the several labour-saving methods for counting reticulocytes reviewed by Dacie and Lewis (1963) the least laborious is the Miller count described by Brecher and Schneiderman (1950). For this method the Miller graticule, a microscope eyepiece graticule with a square of side 7 mm. with a square of one ninth the area in one corner, is used and consecutive fields are counted, reticulocytes in...

A flow cytometry method for rapid detection and enumeration of total bacteria in milk.

Application of flow cytometry (FCM) to microbial analysis of milk is hampered by the presence of milk proteins and lipid particles. Here we report on the development of a rapid (/= 0.98) between the FCM assay and the more conventi...

A method for counting acid-fast bacteria.

A reliable, convenient, and accurate method for counting ac id-fast hacteria ( AFB) is essential for much work with Mycobacterium leprae. The procedure used , previously in the laboratory (~, 4 ) had two technical difficulties: (a) In order for the drops to spread satisfactorily the microscope slides had to have a uniform wetability of the correct degree; sometimes this requirement was difficul...

A Quantitative Differential Method for Counting Mixed Cultures of Bacteria.

New method for counting bacteria associated with coral mucus.

The ability to count bacteria associated with reef-building corals in a rapid, reliable, and cost-effective manner has been hindered by the viscous and highly autofluorescent nature of the coral mucus layer (CML) in which they live. We present a new method that disperses bacterial cells by trypsinization prior to 4',6-diamidino-2-phenylindole (DAPI) staining and quantification by epifluorescenc...